Ovarian Stimulation

  • The ovaries will be stimulated to produce eggs.
  • Firstly, a gonadotropin-releasing hormone (GnRH) analogue is given for about 8-10 days. 
  • Secondly, daily injections of human menopausal gonadotrophin (hMG) are given to stimulate the ovaries to produce an increased number of follicles containing the eggs.
  • After about 8 days, the number and size of the follicles will be measured using ultrasound. 
  • When 1-2 of the follicles reach 18mm in diameter, an injection of human chorionic gonadotrophin is given, and the oocyte retrieval scheduled for 36hrs later.
 

Oocyte Retrieval

  • The oocytes will be retrieved via transvaginal ultrasound.
  • Ultrasound allows the physician to visualize the follicles and can then push a needle into each of them and aspirate the fluid inside the follicle containing the oocyte. 
  • The follicular fluid is examined by laboratory personnel for the presence of the egg and if found, is placed in an incubator.
  • This is done for all the follicles.
 

Semen Processing and Insemination

  • At the time of oocyte retrieval or immediately thereafter, the male partner's sperm will be processed to isolate the highest quality sperm.
  • The best quality sperm will then be used for inseminating the oocytes.
  • Approximately 5-6hrs after the oocyte retrieval, the oocytes and sperm are put together in a petri dish and placed in an incubator.
 

Fertilization Check (Day 1)

  • After 18hours of coincubation, the eggs are examined for the presence of two pronuclei, one male and one female, which will indicate that fertilization has taken place.
  • The fertilized oocytes are then transferred to special culture media to allow them to develope further.
 

Cell Division (Day 2)

  • At this stage, the fertilized oocytes would have started cell division and would show 2 to 4 cells stages.
  • These embryos are then again placed in special media to help them develop further.
 

Embryo Biopsy (Day 3)

  • On Day 3, these embryos will be at the 6-8 cell stage.
  • At this point, it would be considered safe to remove one of the cells (blastomeres) for Preimplantation Genetic Diagnosis (PGD).
  • A hole is made in the outer covering of the embryo (zona pellucida) using an acid solution.
  • Thereafter, one of the blastomeres is aspirated through the hole into a biopsy pipette.
  • The blastomere is placed on a slide and is ready for PGD.
  • The embryos are placed back into the special culture media to allow them to developm futher.
 

Cell Division (Day 4)

  • On Day 4, the embryos will start to compact and enter the morula stage.
 

Embryo Transfer (Day 5)

  • On Day 5, the embryos will be at the blastocyst stage, the last stage before they start to hatch and subeseqnetly impnat into the uterus.
  • Upon review of the Preimplantation Genetic Diagnosis (PGD) results, only the normal embryos will be transferred into the uterus.
  • Embryo transfer involves aspirating the embryos (blastocysts) into a catheter, and passing the catheter through the cervix into the uterine cavity and depositing the embryos in the uterus.
  • The embryos should then be able to implant into the wall of the uterus.
  • Hormonal treatments are given for the following 3 weeks.
  • Any excess embryos not transferred  may be cryopreserved for later use
 

Pregnancy Test

  • A pregnancy test is scheduled for 14 days after the embryo transfer.
 

 

 


310 South Limestone Street, Lexington, KY 40503 USA
P.O. Box 23777 " Lexington, KY 40523 USA
Phone: (859) 254-8108, (859) 226-7263, (859) 226-7264
Fax: (859) 226-0026
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